Antioxidant activity of Methanolic Extracts of Sweet Lime (Citrus lemitta) Peel, Pulp, and Seed by 2,2-diphenyl-1-picryl-hydrazyl free radical.

Abstract

Abstract:

Sweet lime (Citrus lemitta) fruits were collected from a local market in Lahore Pakistan, their peels, pulp, and seed were separated manually and dried under shade for 20 days. After drying, their extracts were prepared separately by immersing each component in methanol at a ratio of 1:5 (w/v) for 14 days at ambient conditions. The antioxidant activity of each extract was evaluated by using 2,2-diphenyl-1-picryl-hydrazyl radical. Butylated hydroxyl toluene was used as a standard, and the percentage inhibition was determined for each extract. The results indicate that peel methanolic extract with concentrations of 25µL, 50µL, 75µL and 100µL has DPPH inhibition of 70%, 74.3%, 79.1%, and 81.2% respectively. Pulp methanolic extract with concentrations of 25µL, 50µL, 75µL and 100µL have DPPH inhibition 65.3%, 69.7%, 74.1%, and 77.1% respectively. Seed methanolic extract with the same concentration has DPPH inhibition 46.2%, 49.7%, 54.6%, and 58.9%respectively. The antioxidant activity of all the extracts was concentration-dependent.